Gel, Affinity Chromatography: Handwritten Notes
Subject: Instrumental Methods of Analysis
Semester: Seventh Semester (Sem 7)
Course: Bachelor of Pharmacy (BPharm)
Keywords: Gel Chromatography, Affinity Chromatography, Handwritten Notes, Instrumental Methods of Analysis, BPharm Notes, Download PDF, Study Material
Gel, Affinity Chromatography: Handwritten Notes
Introduction:
Chromatography is a widely used analytical technique in chemistry, biology, and pharmaceutical sciences for separating, identifying, and purifying components of a mixture. Among various types of chromatography, Gel Chromatography and Affinity Chromatography are two essential methods that play a pivotal role in research and industrial applications. These techniques are extensively covered in the subject Instrumental Methods of Analysis, which is a core part of the seventh semester curriculum for students pursuing a Bachelor of Pharmacy (BPharm).
Gel Chromatography:
Gel Chromatography, also known as Size Exclusion Chromatography (SEC), is based on the principle of separation according to the size of molecules. The stationary phase consists of porous beads, and the mobile phase carries the sample through the column. Larger molecules cannot penetrate the pores and elute faster, while smaller molecules enter the pores and take longer to pass through the column. This method is particularly useful for separating proteins, polymers, and other macromolecules.
Key features of Gel Chromatography include:
- Non-destructive separation process.
- Useful for determining molecular weight distribution.
- Applications in protein purification and polymer analysis.
Affinity Chromatography:
Affinity Chromatography is a highly specific separation technique that exploits the biological interactions between molecules. In this method, the stationary phase contains ligands that bind specifically to the target molecule in the sample. After washing away unbound substances, the target molecule is eluted using a suitable buffer or competitive ligand. This technique is widely used in biochemistry and pharmaceuticals for purifying enzymes, antibodies, and other biomolecules.
Advantages of Affinity Chromatography include:
- High specificity and selectivity.
- Efficient purification of target molecules.
- Applications in drug discovery and biotechnology.
Importance in BPharm Curriculum:
For BPharm students, understanding these chromatographic techniques is crucial as they form the foundation of modern pharmaceutical analysis. Gel and Affinity Chromatography are integral to drug development, quality control, and research in pharmacology. By studying handwritten notes on these topics, students can gain deeper insights into the theoretical concepts and practical applications of these methods.
How to Use These Notes:
The provided handwritten notes are designed to simplify complex topics and make them easier to understand. They include diagrams, step-by-step explanations, and key points that align with the syllabus of Instrumental Methods of Analysis. Students can use these notes for exam preparation, assignments, and practical lab work.
Conclusion:
Gel and Affinity Chromatography are indispensable tools in the field of pharmaceutical sciences. Mastering these techniques not only enhances academic performance but also prepares students for real-world challenges in the pharmaceutical industry. Download the notes to access comprehensive study material tailored for BPharm students.
Info!
If you are the copyright owner of this document and want to report it, please visit the copyright infringement notice page to submit a report.
