Pharmacognocy 2 Unit 3: Phytoconstituents Analysis (Handwritten Notes)
Download comprehensive PDF notes on isolation, identification and analysis of key phytoconstituents. Includes detailed methods for Terpenoids, Glycosides, Alkaloids and Resins. Perfect for pharmacy students seeking handwritten notes, study materials and PPT presentations on phytochemical analysis techniques.
Keywords: phytoconstituents PDF download, alkaloid isolation methods, terpenoid analysis notes, glycoside identification techniques, herbal drug analysis, pharmacognosy handwritten notes, phytochemistry PPT, natural product chemistry.
Phytoconstituents Isolation and Analysis Techniques
1. Terpenoids Analysis
- Menthol: Steam distillation & GC-MS analysis from mint oils
- Citral: Column chromatography isolation from lemongrass
- Artemisinin: HPLC quantification in Artemisia annua
2. Glycosides Study
- Glycyrhetinic Acid: Acid hydrolysis followed by TLC validation
- Rutin: UV-spectrophotometric quantification in buckwheat
3. Alkaloid Isolation
- Atropine: Soxhlet extraction from Belladonna roots
- Quinine: Column chromatography from Cinchona bark
- Reserpine: pH-dependent extraction from Rauwolfia
- Caffeine: Supercritical CO₂ extraction from tea leaves
4. Resin Analysis
- Podophyllotoxin: Flash chromatography from Mayapple resin
- Curcumin: Spectrofluorometric analysis in turmeric
Analytical Techniques Covered:
- Thin Layer Chromatography (TLC) for preliminary screening
- High Performance Liquid Chromatography (HPLC) for quantification
- Gas Chromatography-Mass Spectrometry (GC-MS) for volatile compounds
- Nuclear Magnetic Resonance (NMR) for structural elucidation
- UV-Vis Spectroscopy for concentration determination
The notes provide 500+ words of detailed protocols, safety precautions, and quality control parameters for each analytical method, along with industrial applications of these techniques in phytopharmaceutical manufacturing.
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